Reviewer name and names of any other individual's who aided in reviewer	Chaochao Yan
Do you understand and agree to our policy of having open and named reviews, and having your review included with the published papers. (If no, please inform the editor that you cannot review this manuscript.)	Yes
Is the language of sufficient quality?	Yes
Please add additional comments on language quality to clarify if needed
Are all data available and do they match the descriptions in the paper?	No
Additional Comments	In the section "Availability of supporting data," it is stated that "supporting datasets, including annotation, are available at GigaDB." However, I was unable to locate these datasets during my search. Could you please provide a direct link or the accession number to access these resources?
Are the data and metadata consistent with relevant minimum information or reporting standards? See GigaDB checklists for examples <a href="http://gigadb.org/site/guide" target="_blank">http://gigadb.org/site/guide</a>	Yes
Additional Comments
Is the data acquisition clear, complete and methodologically sound?	No
Additional Comments	The manuscript currently lacks detailed information regarding the samples and data used to assemble and annotate the reference genome. For instance, it does not specify how many samples or libraries were used for RNA-Seq or whole-genome sequencing. I suggest including a table that provides comprehensive information on the samples and sequencing data. Additionally, while the manuscript mentions that "Genomic DNA was sequenced using the Illumina Novaseq 6000 platform," the corresponding Illumina data are not described. I am unclear about how the PacBio CCS reads were produced. Could you please provide more details or clarify the methodology used to generate these reads?
Is there sufficient detail in the methods and data-processing steps to allow reproduction?	No
Additional Comments	Some methods described in the manuscript lack sufficient detail, particularly for tools such as BLAST, BlobTools, HiRise, and BWA. To ensure reproducibility, I recommend providing the specific parameters used for these analyses.
Is there sufficient data validation and statistical analyses of data quality?	Yes
Additional Comments
Is the validation suitable for this type of data?	Yes
Additional Comments
Is there sufficient information for others to reuse this dataset or integrate it with other data?	Yes
Additional Comments
Any Additional Overall Comments to the Author
Recommendation	Minor Revision