(A) Comparison of mean cycle thresholds between RT-qPCR and qPCR according to different invasive aspergillosis classification modalities. (B) Corresponding mean ΔCt, computed as the difference between the Ct obtained in qPCR and that obtained in RT-qPCR. IA classification was performed using the EORTC-MSGERC criteria. In Fig. 1A, the middle line of the boxplot indicates the median, and the cross indicates the mean. The bar indicates the minimum and maximum values. In Fig. 1B, the bar indicates the CI95% of the mean ΔCt. ns, not significant, ****: P < 0.0001. Abbreviation: EORTC-MSGERC, European Organisation for Research and Treatment of Cancer and Mycoses Study Group Education and Research Consortium; IA, invasive aspergillosis.