TABLE 2.
Analytical performance of the RT-qPCR and qPCR for the diagnosis of invasive aspergillosis according to clinical classification and sample typea,b
| All classifications (possible, probable, proven) | Probable | Probable and proven | |||||
|---|---|---|---|---|---|---|---|
| RT-qPCR | qPCR | RT-qPCR | qPCR | RT-qPCR | qPCR | ||
| All samples | Se | 0.66 ** [0.56–0.74] |
0.49 [0.39–0.58] |
0.87 * [0.75–0.93] |
0.74 [0.60–0.84] |
0.86 [0.74–0.93] |
0.73 [0.60–0.83] |
| Sp | 0.79 [0.70–0.86] |
0.91 * [0.83–0.95] |
0.79 [0.69–0.86] |
0.91 * [0.84–0.96] |
0.79 [0.69–0.86] |
0.91 * [0.84–0.95] |
|
| Plasma samples | Se | 0.34 *** [0.19–0.53] |
0.00 [0.0–0.12] |
0.43 * [0.16–0.75] |
0.00 [0.0–0.35] |
0.38 * [0.14–0.69] |
0.00 [0.0–0.32] |
| Sp | 0.81 [0.63–0.92] |
1.0 ** [0.88–1.0] |
0.81 [0.63–0.92] |
1.00 ** [0.88–1.00] |
0.81 [0.63–0.92] |
1.00 ** [0.88–1.00] |
|
| Respiratory samplesc | Se | 0.78 [0.67–0.86] |
0.71 [0.59–0.80] |
0.95 [0.85–0.99] |
0.91 [0.78–0.96] |
0.95 [0.85–0.99] |
0.91 [0.79–0.96] |
| Sp | 0.85 [0.68–0.91] |
0.90 [0.77–0.96] |
0.80 [0.66–0.89] |
0.90 [0.77–0.96] |
0.83 [0.68–0.91] |
0.90 [0.77–0.96] |
|
a
Sensitivity (Se) and Specificity (Sp) are provided with their 95% confidence intervals [95% CI].
b
*: significantly superior with *: P < 0.05, **: P < 0.01, ***: P < 0.001.
c
Respiratory sample includes bronchoalveolar fluid, bronchial aspirate, tracheal aspirate, and sputum.