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. 2024 Oct 18;15(11):e02611-24. doi: 10.1128/mbio.02611-24

Fig 4.

Dose-dependent binding curves of amphotericin B, gladiolin, or their combination to model membranes. Amphotericin B shows a quick and reversible binding while gladiolin’s binding is slower and partially irreversible.

Gladiolin interacts with model membranes and modulates membrane binding by AmpB. Dose-response analysis of (A) AmpB or (B) gladiolin binding to model lipids. Sensorgrams were obtained from POPC or POPC:ergosterol model lipids with ratios of either 90:10 or 80:20. The drugs were injected in twofold dilution series ranging from 0 to 16 µg/mL. (C) SPR using concentrations of AmpB from 0.5 to 16 µg/mL with a fixed concentration of 2 µg/mL gladiolin. (D) Same as panel C, but here, gladiolin concentrations varied from 0.5 to 16 µg/mL with a fixed concentration of 2 µg/mL AmpB. The sensorgrams shown are a representative example of three independent replicates.