TABLE 1.
Bacterial strains and plasmids used in this study
| Strain name | Genotype or function | Reference for allele or plasmid |
|---|---|---|
| S. aureus USA300_LAC strains | ||
| JMB1100 | USA300_LAC wild type (WT) | Alexander Horswill |
| JMB8689 | Δfpa::tetM | This study |
| JMB8428 | fpa::Tn | (50) this study |
| JMB1432 | Δfur::tetM | (51) |
| JMB4536 | hemB::Tn | (52) |
| JMB10638 | Δfpa::tetM fur* | This study |
| JMB10675 | proC::Tn | (50) and this study |
| JMB10677 | Δfpa::tetM proC::Tn | (50) and this study |
| JMB10676 | fur* proC::Tn | (50) and this study |
| JMB10678 | Δfpa::tetM fur* proC::Tn | (50) and this study |
| JMB7525 | fhuC::Tn | (6) |
| JMB10664 | Δfur::tetM fhuC::Tn | |
| JMB10721 | Δfpa::tetM fhuC::Tn | (50) and this study |
| JMB10722 | Δfpa::tetM fur* fhuC::Tn | (50) this study |
| JMB10641 | fpa::TnΔfur::tetM | This study |
| JMB 9738 | geh::suf _hld RBS_lacZ | (53) |
| B. subtilis strains | ||
| BD630 | hisA1 leuA8 metB5 | (54) |
| BD8592 | hisA1 leuA8 metB5 Phyperspank_yfp_fpa | This study |
| BD8012 | hisA1 leuA8 metB5 Phyperspank_gfp | (55) |
| VCB102 | BD8592 Dfpa::pMutin4 | This study |
| VCB103 | hisA1 leuA8 metB5 Dfpa::pMutin4 | This study |
| Plasmids | ||
| pEPSA5 | Inducible expression complementation | (56) |
| pEPSA5_fpa | Inducible expression complementation | This study |
| pCM28 | Native promoter complementation | (57) |
| pCM28_fpa | Native promoter complementation | This study |
| pJB38 | Mutant generation | (58) |
| pJB38_Δfpa::tetM | Mutant generation | This study |
| pDR111_YFP | Protein interaction studies | (59) |
| pED2143 | Protein interaction studies | This study |
| pMutin4-fpa | Knockout construct | This study |
| pOS_saeP1_gfp | Transcriptional reporter | Shaun R. Brinsmade |
| pOS_isdC_gfp | Transcriptional reporter | This study |
| pOS_fhuA_gfp | Transcriptional reporter | This study |
| pOS_sbnA_gfp | Transcriptional reporter | This study |
| pOS_saeP1_lacZ | Transcriptional reporter | This study |
| pOS_isrR_lacZ | Transcriptional reporter | This study |
| pOS-1-plgt | Genetic complementation lgt promoter | (60) |
| pOS-1-plgt_fur pGEX-6P1_fpa pGEX-6P1_fur | Genetic complementation lgt promoter Expression of fpa Expression of fur |
(2) This study This study |
| pSmBIT | Split luciferase assay | (61) |
| pLgBIT pSmBIT_fpa | Split luciferase assay Split luciferase assay |
(61) This study |
| pSmBIT_fur pLgBIT_fur | Split luciferase assay Split luciferase assay |
This study This study |
| pLgBIT_fpa | Split luciferase assay | This study |