Fig. 5. Importin-mediated HBV capsid interaction with NuPODs.

(A) HBV capsids mixed with capped 60-nm Nup62 NuPODs in the presence of 100 nM importin-β1 (impβ). (B) HBV capsids mixed with capped 60-nm Nup153 NuPODs in the presence of 100 nM impβ. (C) HBV capsids mixed with capped 60-nm Nup62-Nup153 NuPODs in the presence of 100 nM impβ. For (A) to (C), schematic diagrams of the binding experiments are shown next to representative TEM images. The experiments were repeated twice (technical replicates) with similar results. Scale bar, 100 nm. (D) Percentages of NuPODs occupied by HBV capsids with (green) or without (gray) 100 nM impβ. NuPODs counted in each experiment (from left to right): 288, 206, 168, 380, 279, and 203. Occupancies of importin-free Nup153 and Nup62-Nup153 NuPODs (Fig. 4E) are shown here for comparison.