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. 2024 Nov 13;10(46):eadq9183. doi: 10.1126/sciadv.adq9183

Fig. 2. Juvenile starvation controls sexually dimorphic PHB>AVA synaptic contacts via CRH-1/CREB.

Fig. 2.

(A) Quantification of PHB>AVA synaptic GRASP(otIs839) in well-fed wild-type animals and L1-starved wild-type and animals expressing PHB::GOA-1gof(otEx7925 and otEx8158). (B) Quantification of PHB>AVA synaptic GRASP(otIs839) in wild type and tph-1(ot1274) in both sexes. (C) Quantification of PHB>AVA synaptic GRASP(otIs839) in wild-type and tph-1(ot1274) males expressing PHB::GOA-1gof(otEx7925 and otEx8158). (D) Quantification of PHB>AVA synaptic GRASP(otIs839) in wild-type and crh-1(tz2) and crh-1(ot1342) animals of both sexes. (E) Quantification of PHB>AVA synaptic GRASP(otIs839) in wild-type and crh-1(tz2) males expressing PHB::GOA-1gof(otEx7925 and otEx8158). (F and G) Quantification of PHB>AVA synaptic GRASP(otIs839) in crh-1(tz2) (F) and tph-1(ot1274) (G) males with overexpressing CRH-1 missense allele transgenes (otEx8045 for CRH-1WT, otEx8046 for CRH-1S48E, and otEx8082 for CRH-1S48A) in the PHB neurons. WT, wild type. (H) Schematic diagram indicates juvenile food experience acts through serotonin–G protein–coupled receptor–GOA-1 to activate CRH-1 to secure LIN-29A expression upon sexual maturation to establish PHB>AVA sexually dimorphic connectivity. Statistics: [(A), (C), and (E) to (G)] One-way ANOVA and [(B), (D), and (I)] two-way ANOVA followed by Bonferroni multiple comparisons test. P value and n numbers are indicated on the graph. Scale bars, 10 μm. + indicates the mean value.