Fig 8. Trypsin treatment modulates sarbecovirus neutralization by the pan-sarbecovirus monoclonal antibody S2H97.

Particles bearing the indicated S proteins were preincubated with or without trypsin (50 μg/ml for 30 min at 37°C) and subsequently trypsin inhibitor (200 μg/ml for 10 min at 37°C). Thereafter, the particles were incubated with different concentrations of the pan-sarbecovirus monoclonal antibody S2H97 (30 min at 37°C) before being added to Vero-ACE2-TMPRSS2 cells. S protein-driven cell entry was analyzed by measuring the activity of virus-encoded firefly luciferase in cell lysates at 16-18h post inoculation and normalized to entry in the absence of antibody. Presented are the average (mean) data of three biological replicates, each performed with four technical replicates. Error bars indicate SEM. Statistical significance was assessed by two-way analysis of variance with Sidak’s multiple comparisons test (p > 0.05, not significant [ns]; p ≤ 0.05, *; p ≤ 0.01, **; p ≤ 0.001, ***).