Figure 5.

ORF6_CTR is very dynamic in both the trans-P57 and cis-P57 conformations. (A) ¹⁵N longitudinal relaxation rates (R₁), (B) ¹⁵N transverse relaxation rates (R₂), (C) {¹H}-¹⁵N steady-state hetNOEs, and (D) J(0) values of 300 μM ¹⁵N-labeled ORF6_CTR at 15°C and pH 6.9. The backbone motions are shown for all peaks corresponding to the trans-P57 conformation (green), and only for the well-resolved peaks with a measurable intensity corresponding to the cis-P57 (pink) conformation. Error bars represent the fitting of the NMR data in (A) and (C). The error bars for (B) were calculated by error propagation. The error bars for (D) represent the standard deviations for each residue, which were calculated using Monte Carlo uncertainty propagation. All experiments were recorded at a static magnetic field strength of 14.1 T.