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. 2024 Oct 31;15:1474516. doi: 10.3389/fimmu.2024.1474516

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Ziehl-Neelsen stain of the gut of flies treated with heat-killed M. manresesis. Both male and female flies contain acid-fast bacteria in their guts 24- (A) and 48-hours (B) after the start of the treatment. Arrows point to the M. manresensis clusters in the samples, and their presence confirms oral treatment effectiveness. Pictures taken with the NIS-Elements D Software (Nikon Instruments Inc, version 5.11.03).