Effects of EPS8 overexpression on cell proliferation and viability. A. Cells transfected with pCMV-EPS8 exhibited higher levels of cell proliferation when compared with untreated cells and those transfected with the pCMV-GFP based on the MTT assay. B. Cells transfected with pCMV-EPS8 exhibited higher levels of cell viability when compared with untreated cells and those transfected with pCMV-GFP based on the CCK-8 assay. *P < 0.05 vs. Control and #P < 0.05 vs. pCMV-GFP. CCK-8, Cell Counting Kit-8; Enz-R, enzalutamide-resistant; GFP, green fluorescent protein; MMT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.