Figure 3.

Effects of EPS8 overexpression on the EMT. Western blotting revealed that LNCaP and LNCaP EnzaR cells transfected with pCMV-EPS8 exhibited lower levels of E-cadherin expression and higher levels of α-SMA, Snail, and Slug when compared with untreated cells and those transfected with pCMV-GFP. (A, C) LNCap cells and (B, D) LNCaP Enz-R cells. The triplicates of western blotting images are shown in Figures S2 and S3. *P < 0.05 vs. Control and #P < 0.05 vs. pCMV-GFP. α-SMA, α-smooth muscle actin; Enz-R, enzalutamide-resistant; GFP, green fluorescent protein.