Figure 4.

Effect of EPS8 knockdown on cell proliferation and viability. A. Cells transfected with shRNA-EPS8 exhibited higher levels of cell proliferation when compared with untreated cells and those transfected with shRNA-control based on the MTT assay. B. Cells transfected with shRNA-EPS8 revealed lower levels of cell viability when compared with untreated cells and those transfected with shRNA-control based on the CCK-8 assays. *P < 0.05 vs. Control and #P < 0.05 vs. pCMV-GFP. CCK 8, Cell Counting Kit 8; Enz-R, enzalutamide resistant; MMT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; shRNA, short-hairpin RNA.