Figure 5.

Effects of EPS8 knockdown on the EMT. Western blotting revealed that LNCaP and LNCaP Enz-R cells transfected with shRNA-EPS8 exhibited increased E-cadherin expression and reduced α-SMA, Snail, and Slug expression when compared with untreated cells and those transfected with shRNA-control. A. Western blotting images of EMT markers in the LNCaP cells. B. Quantification of EMT markers expression in LNCaP cells. C. Western blotting images of EMT markers in the LNCaP Enz-R cells. D. Quantification of EMT markers expression in LNCaP Enz-R cells. The triplicates of western blotting images are shown in Figures S4 and S5. *P < 0.05 vs. Control and #P < 0.05 vs. pCMV-GFP. α-SMA, α-smooth muscle actin; EMT, epithelial-to-mesenchymal transition; Enz-R, enzalutamide-resistant; GFP, green fluorescent protein; shRNA, short-hairpin RNA.