Figure 8.

Immunohistochemical evaluation of EMT marker expression in xenograft tumors. LNCaP xenograft tumors were analyzed for the expression of (A) EPS8, (B) α-SMA, and (C) E-cadherin by IHC after 25 days of transfection. (D) Higher EPS8, higher α-SMA, and lower E-cadherin levels were detected in the pCMV-EPS8-transfected xenograft tumor cells. Conversely, lower EPS8, lower α-SMA, and higher E-cadherin levels were detected in the shRNA-EPS8-transfected xenograft tumor cells. #P < 0.05 vs. Control, *P < 0.05 vs. pCMV-GFP, + P < 0.05 vs. shRNA-control. α-SMA, α smooth muscle actin; EMT, epithelial-to-mesenchymal transition; GFP, green fluorescent protein; IHC, immunohistochemistry; shRNA, short-hairpin RNA.