Extended Data Fig. 15. Increased spatial tuning of hippocampal PV interneurons after removal of CP-AMPARs.

a, Raster plots of Ca²⁺ activity over distance (normalized) for PV interneurons expressing GFP (left) or SEP-GluA2 (right). Activity for cells with significant spatial tuning is shown on top (magenta, green, respectively) and sorted by the peak location of activation. Activity of non-spatially tuned cells is shown below (greyscale). b, Average cross correlation of spatial activity profiles of cells between the first and second half of recording sessions (10 runs each). Note the higher and more consistent correlations of spatial tuning in cells expressing GluA2. c, Left, two representative examples of spatial vector tuning for a GFP (magenta) and SEP-GluA2 expressing (green) PV interneuron. The cell’s activity along the track is projected into a circular coordinate system. The orientation and length of the calculated tuning vector in the center correspond to the tuning direction and specificity, respectively. Right, Average activity profile for all GFP (magenta) or SEP-GluA2 expressing (green) PV interneurons aligned to their preferred orientation (0°). Note the higher asymmetry and stronger vectorial tuning for GluA2 expressing cells. d, Fraction of cells with significant spatial tuning in GFP (magenta) and SEP-GluA2 (green) expressing animals. Dots denote fractions in individual experiments. (n = 8/8 experiments from n = 4/4 mice, P = 0.001, t-test). e, Spatial information normalized to activity (see Methods) for GFP (magenta) and SEP-GluA2 (green) expressing cells. Dots denote individual cells. (n = 583/476 cells from n = 4/4 mice, P = 2.23×10⁻⁸, Wilcoxon rank-sum test). f, Same as in (e) but for spatial map stability (correlation) between the first and second half of the recording session (P = 0.0026, Wilcoxon rank-sum test). g, Same as in (e) but for spatial activity map correlations between individual trials of a session (P = 2.28×10⁻⁹, Wilcoxon rank-sum test). Note the consistently higher stability of spatial representation in PV interneurons expressing GluA2. Black lines in (e-g) denote mean ± SEM, and the red dotted line denotes the median. Dots denote values for individual cells.