Fig. 4. H2S donors screening assay based on TRES technique.
After being treated with the 320 natural products (10 μM) from the Traditional Chinese Medicine Monomer Library or DMSO in cells for 1 h, Ir-mito1 probe (10 μM) was further incubated for another 1 h. The luminescence signal output in cells was recorded using the TRES technique. The luminescence signal of Ir-mito1 in different treatment groups was compared with the DMSO control group. The means of the results are calculated from three independent experiments.