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. 2024 Nov 13;14:27890. doi: 10.1038/s41598-024-79409-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — hnRNPAB X2 dynamically associates with L-bodies via its RBD and IDR. (a) Shown is an image of the vegetal cytoplasm of a stage II oocyte expressing mCh-hnRNPAB X2. FRAP was conducted such that an individual L-body was partially bleached (a′), to allow for recovery (a′′) both from within the L-body and from its environment. The 10 μm² ROI is indicated by a white box; scale bar = 10 μm. (b) Stage II oocytes were microinjected with mCherry (mCh), mCh-hnRNPAB X2, mCh-RBD or mCh-IDR RNA to express the mCh-tagged proteins, along with Cy5 LE RNA to mark L-bodies. Normalized FRAP recovery curves are shown (n = 21 oocytes per fusion protein); error bars represent standard error of the mean. Measurements were taken at 5 s intervals over 100 iterations. (c) Plateau values (% mobile fraction) are shown for mCh-hnRNPAB X2 (green, 96%±0.013), mCh-RBD (blue, 98%±0.014), mCh-IDR (orange, 98%±0.002), and mCherry (red, 104%±0.29). Error bars represent standard error of the mean. Statistics shown are an Ordinary one-way ANOVA with Tukey’s multiple comparisons; ns indicates not significant (p > 0.05) and * indicates p = 0.04. (d) T_1/2 measurements are shown for mCh-hnRNPAB X2 (green, 30.1s ± 3.4), mCh-RBD (blue, 9.3s ± 0.4), mCh-IDR (orange, 10.1s ± 0.6), and mCherry (red, 6.0s ± 1.6), measured as the average time at which the protein recovers half of its plateau value. Error bars represent standard error of the mean. Statistics shown are an Ordinary one-way ANOVA with Tukey’s multiple comparisons; ns indicates not significant (p > 0.05), *** indicates p < 0.001, **** indicates p < 0.0001.