Fig. 3.

Thiol-Michael addition hydrogel synthesis. A The synthetic hydrogel was injected and crosslinked directly into the agarose resection cavity by using the dual chamber syringe mixer. B Synthetic hydrogel bio-orthogonality. In triplicate, synthetic hydrogels were injected into 1X PBS. PEGDA precursor solutions were mixed with blue food color dye to visualize the hydrogel during crosslinking. Hydrogels were crosslinked at 37 °C and sol–gel transition was assessed qualitatively.