Table 1.

Information about the primers and probe used in this study for DNA barcoding and to specifically detect parasitoids in wheat midges.

Application	Gene	Specificity	Name	Sequence	Thybr	Size (pb)	References
DNA barcoding	COI	–	C1-J-1718F	GGAGGATTTGGAAATTGATTAGTTCC	47 °C	463–472	Simon et al.28
		–	C1-N-2191	CCCGGTAAAATTAAAATATAAACTTC			Simon et al.28
	18S	–	18S-441 F	AAATTACCCACTCCCGGCA	50 °C	774–828	Heraty et al.29
		–	18S-1299 R	TGGTGAGGTTTCCCGTGT T			Heraty et al.29
	28S	–	28S-F	AGAGAGAGTTCAAGAGTACGTG	55 °C	367–489	Dowton and Austin30
		–	28S-R	TTGGTCCGTGTTTCAAGACGGG			Dowton and Austin30
Taqman detection of parasitism	18S	Parasitoid-group	Taqman probe/ParTaq536	FAM- CTTGGATCGTCGCAAG-MGB	60 °C	145	This study
		Parasitoid-group	Par500F	CCGAG(G/A)TAATGATTAATAGGGACAGA
		-	Par643R	CGAACCTCTAACTTTCGTTCTTGA
Group specific detection	18S	Parasitoid-group	Par164F	AAGCTCGTAGTTGAATCTGTG	60 °C	302–358	This study
		Parasitoid-group	Par473R	CCCCCATCTGTCCCTATTA
		Midge-group	Cec164F	ACGTTCGTAGTTGAACTTGTG	60 °C	326	This study
		Midge-group	Cec473R	CCCCCAATTGCCTCCATTA
Species specific detection	18S	Parasitoid-group	Par164F	AAGCTCGTAGTTGAATCTGTG			This study
		M. penetrans	macro382R	CAGTATTCAGGCGAACATAG	60 °C	214
		E. error	euxe382R	GATTTTTCAGGCTTTTGTTAGG	60 °C	246
		P. tuberosula	tuber295R	TAAAGCTCCCAACGAGACGA	60 °C	123
		P. equestris	Equation 382R	GAATTTTCAGGCTTTTCAATTG	55 °C	239
		P. gracilipes	graci382R	GAATTTTCAGGCGTATATTTTG	55 °C	233
		S. myles	sinop382R	GATAATTCAGGCTTGTTGTAGG	55 °C	270

T_hybr: optimal hybridisation temperature.