Table 4.
Detection of midge parasitism at seven locations in Belgium.
| Location | Latitude N | Longitude E | N | Parasitised | % | M. Penetrans | P. tuberosula | E. error | M. Penetrans + E. error |
|---|---|---|---|---|---|---|---|---|---|
| Ciney | 50,30725252 | 5,06835821 | 27 | 4 | 14.8 | 3 | 0 | 0 | 1 |
| Ernage | 50,60288404 | 4,67571176 | 27 | 4 | 14.8 | 0 | 1 | 3 | 0 |
| Gembloux | 50,58368337 | 4,69034771 | 16 | 4 | 25.0 | 3 | 1 | 0 | 0 |
| Juprelle | 50,70804044 | 5,58654127 | 16 | 2 | 12.5 | 1 | 1 | 0 | 0 |
| Lillois | 50,62194227 | 4,37769566 | 15 | 0 | 0.0 | 0 | 0 | 0 | 0 |
| Limont | 50,66634385 | 5,32071588 | 15 | 5 | 33.3 | 5 | 0 | 0 | 0 |
| Sorée | 50,40837572 | 5,12478659 | 16 | 1 | 6.3 | 1 | 0 | 0 | 0 |
| Total | 132 | 20 | 15.2 | 13 | 3 | 3 | 1 |
Parasitism of S. mosellana larvae was assessed by using the TaqMan method and the group specific PCR assay developed in this study. Species were identified by Sanger sequencing and species-specific PCR amplification. N: sample size.