Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Nov 13;14:27867. doi: 10.1038/s41598-024-79217-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Crown 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Electrophoretic profiles of VPSPr129-MM fragments and their sensitivity to proteinase-K digestion. (A) Mock “western blot view” of VPSPr129-MM digested with 25 µg/ml of PK treated with or without PNGase using the CE assay. Fragments are labelled according to convention described in Figure 2. (B) Electrophoretogram of VPSPr129-MM digested with 25 µg/ml of PK (C) Electrophoretogram of PK-titration assay of a VPSPr129-MM case. The arrows demonstrate the shift from a higher molecular weight to a lower molecular weight due to the truncation of fragments as PK concentrations increase (3G → 2G) (D) Graph showing the PrP signal intensity change of each VPSPr fragment throughout the PK titration assay.