Figure 6.

High-content imaging of excitatory and inhibitory neurons co-cultured with mouse glia for iSTOP LoF hiPSC lines

(A) Representative images of neural cultures with hiPSC-differentiated excitatory neurons labeled by tdTomato (red). Neurons were stained for MAP2, SYN1, tdTomato, and DAPI. TdTomato and DAPI staining were used to quantify neurite growth/branches; tdTomato, MAP2, and SYN1 staining were used to analyze synaptic puncta. Scale bar: 20 μm.

(B) Summarized imaging result for neurite outgrowth and branches and synaptic puncta (left to right) for SHANK3 (−/−) and CUL1 (+/−) LoF alleles on donor hiPSC line CW20107. Each data point represents the measurement of an independent well of neuron culture on a 96-well plate; N = 8 wells. The upper limit of the horizontal line is 5%, and the lower limit is 95%; the box has three limites: 25%, 50% and 75%. All %s are fractions as defined by the Box and Whisker plot function in Prism 7.