FIGURE 5.

Pcbp1 was the core gene in macrophage senescence and ferroptosis. (a) Gene expression levels of overlapped ferroptosis‐related genes from single‐cell RNA sequencing data. (b) RT‐qPCR analysis of Pcbp1, Hmox1, and Sat1 mRNAs in drug‐induced senescent macrophage models in vitro. (c) Significant correlations between the Pcbp1, Hmox1, and Sat1 expression level and the GSVA score of reactive oxygen species pathway and interferon‐gamma response pathway in single‐cell RNA sequencing data. (d) Representative western blot and quantification of protein expression of Pcbp1 in drug‐induced senescent macrophage models in vitro. (e) The transfection efficiency of Pcbp1 overexpressed plasmid or pcbp1 siRNA. (f, g) Transfected Pcbp1 siRNA with H₂O₂ and BLM stimulation. Representative western blot and quantification analysis of protein expression of p21 and Gpx4 in macrophages were shown. (h, i) Transfected Pcbp1 overexpression plasmid with H₂O₂ and BLM stimulation. Representative western blot and quantification analysis of protein expression of p21 and Gpx4 in macrophages were shown.