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. 2024 Jul 17;23(11):e14275. doi: 10.1111/acel.14275

FIGURE 6.

FIGURE 6

Stat1 was the key transcription factor in macrophage senescence and ferroptosis by SCENIC analysis. (a) Heatmap showing the age‐related transcription factors in macrophages during aging by SCENIC analysis. (b) The expression of Taf1, Gtf2f1, Yy1, Zmiz1, Etv6, Srebf2, and Stat1 in the single‐cell RNA sequencing data. (c) RT‐qPCR analysis of Stat1 mRNAs in the drug‐induced senescent macrophage models in vitro. (d) Unsupervised regulator analysis showed that Pcbp1 is within the regulon of Stat1. Yellow means up, blue means down. (e) KEGG enrichment analysis of predicted target gene of Stat1. (f) Binding motifs of Stat1 from the JASPAR database. (g) A schematic illustration depicts the Stat1 motif within the promoter region of the Pcbp1 locus. (h) ChIP assay analysis of Stat1 binding to Pcbp1 in macrophages treated with or without BLM. Data were represented as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001.