TABLE 1.
S. No. | Antioxidant assay method | Test sample and standard | Test concentration | IC50 value (μg/mL) | |||
---|---|---|---|---|---|---|---|
0.1 μg/mL | 1 μg/mL | 10 μg/mL | 100 μg/mL | ||||
1. | DPPH free radical scavenging | J. regia extract | 58.20 ± 2.59 | 67.86 ± 0.73 | 88.97 ± 4.72 | 94.71 ± 0.33 | 2.31 |
Standard (ascorbic acid) | 56.83 ± 2.86 | 63.15 ± 5.4 | 95.16 ± 1.97 | 97.83 ± 0.06 | 2.44 | ||
2. | NO free radical scavenging | J. regia extract | 26.98 ± 7.13 | 29.78 ± 6.97 | 37.28 ± 3.59 | 48.85 ± 1.45 | 35.66 |
Standard (curcumin) | 27.09 ± 4.11 | 29.59 ± 4.64 | 33.35 ± 3.45 | 42.22 ± 1.93 | 79.60 | ||
3. | H2O2 radical scavenging | J. regia extract | – | – | – | 28.95 ± 4.51 | – |
Standard (ascorbic acid) | – | – | – | 87.99 ± 0.2 | – |
Note: Average ± SEM (standard error of mean) (n = 3) is used to express all data.