Fig. 2.

PAE prevents cell loss induced by MPP⁺ in SH-SY5Y cells. A, B Cells were pre-incubated with PAE for 2 h followed by treating with or without MPP⁺ (500 μM) for another 48 h, Calcein-PI staining was performed to evaluate the role of PAE on cell death (bar = 100 μm). C, D Cell apoptosis was evaluated by flow cytometry. E–G Expression of Bcl-2, Bax and cleaved caspase-3 was assessed by Western blot. PAE pretreatment attenuated the reduction of Bcl-2/Bax ratio and up-regulation of cleaved caspase-3 induced by MPP⁺ (n = 3). Data are presented as mean ± SD, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001, vs. control group, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001, vs. MPP⁺ treated group