Fig. 6.

PAE can activate AKT/ GSK3β/β-catenin pathway in SH-SY5Y cells. A After pre-incubation with different concentrations of PAE for 2 h, SH-SY5Y cells were treated with or without MPP⁺ (500 μM) for another 48 h. The expression of p-AKT, AKT, p-GSK3β (Ser9), GSK3β and β-catenin was determined by western-blot. PAE treatment reversed MPP⁺-induced down-regulation of p-AKT/AKT ratio (B), p-GSK3β/GSK3β ratio (C) and β-catenin (D). The AKT inhibitor, MK-2206, blocked the effect of PAE on p-AKT/AKT ratio (E, F), p-GSK3β/GSK3β ratio (E, G) and β-catenin (E, H). Results are presented as mean ± SD, n = 3, ^*p < 0.05, ^**p < 0.01, vs. control group, ^#p < 0.05, ^##p < 0.01, vs. MPP⁺ treated group, ^{^}p < 0.05, ^{^^}p < 0.01, vs. MPP⁺ + PAE group