Fig. 3. Inhibitory activity and ligand efficient of non-covalent ADAPTins against the PD1:PDL1 interaction. (A) Mean EC50 values determined from dose-dependent binding curves obtained by ELISA in a PD1–PDL1 assay; n = 3, SD reported in the ESI.† (B) Comparative plot of PD1·PDL1 inhibitory activity by single side chain modulation within ADAPTins. Original loops with Phe/Trp and their Gly-derived analogs. Experimental EC50 values from inhibitory dose–response curves. Error bars indicate the mean ± SD, (n = 3).
