Fig. 6. Ex vivo ADAPTin activity on the PD1 checkpoint pathway. Non-covalent ADAPTin2c/2p were used as negative control. (A) Levels of inflammatory cytokines measured by flow-cytometry (FACS) after 6 h incubation with electrophilic ADAPTins. Error bars indicate the mean ± SD, (n = 3). (B) Rescue of exhausted PBMCs from a melanoma patient. Levels of inflammatory cytokines and granule-associated proteins released (6 h) measured by FACS (healthy and untreated exhausted PBMCs: h-/e-PBMCs used as positive and negative controls respectively). Error bars indicate the avg ± SD, (n = 2).