Library of ADAPTins with diversely functionalized H3 loops generated from an individual CDR-H3 binding analysis. Evaluation of folding and stability properties of the designed ADAPTin peptides 1–2.

^aIndividual CDR buried surface area (BSA) computed by dr_SASA with %-binding surface calculated as a ratio to the total BSA (TBSA) from Ab·PD1 cocrystal structures.

^bTotal number of hydrophobic and polar binding contacts created at the CDR-H3 interface.

^cBinding Gibbs free energy (reu) calculated by Peptiderive to score binding interfaces.

^dFolded fraction (χ_F) at 25 °C and melting temperatures calculated from CD-melts based on the type of ADAPTin fold (B: bulged, R: regular).