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. 2024 Nov 14;29:546. doi: 10.1186/s40001-024-02129-3

Fig. 6.

Fig. 6

ChREBP Knockdown Prevents High Glucose-evoked Microglial Activation Following Exposure to LPS. BV2 cells were exposed to lenti-ChREBP–shRNA (sh-ChREBP) or lenti-NC (NC) for 48 h, and the resulting cell lines were then subjected to screening utilizing purinomycin (5 μg/mL), subsequent treatment with LPS (1 μg/mL) and/or 25.0 mM glucose (HG). ac 12 h after treatment, extracellular lactate content and intracellular glycolytic and mitochondrial ATP levels were assessed. n = 4. dk HIF-1α, HK2, PKM2, IL-1β, TNF-α and HMGB1 expression level. l ChREBP, HIF-1α, HK2, and PKM2 relative mRNA levels (n = 3 independent measurements). Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001