Fig. 5.

Expression patterns of aristaless (al) and BarH1 at different stages of the eutardigrade Hypsibius exemplaris limb development. A, B Stage 14. C, D Stage 16. E, F Stage 17. A–F BarH1 expression. A’–F’ al and BarH1 expression. Figures with the same letter indicates the same embryo viewed in similar optical sections. A, C, E Embryos in lateral view and facing right. B, D, F Embryos in dorsoventral mount. Roman numeral number indicates leg number (e.g., I—1st pair of legs). Nuclei are labeled with DAPI (Gray). Red arrowheads indicate the two patches of BarH1 expression observed at stage 16