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. 2024 Nov 13;22:531. doi: 10.1186/s12916-024-03759-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — Histochemical and immunohistochemical analysis of ECM components of the corneal stroma of control human native corneas (CTR-C), control human native scleral limbi (CTR-L), and human artificial corneas (HAC). Three samples were analyzed per type of tissue (n = 3). VHF, Verhoeff histochemistry for elastic fibers; RET, Gomori’s reticulin histochemistry for the identification of reticular fibers; PSR, picrosirius red histochemical method for collagen fibers; COL-I, immunohistochemistry for type-I collagen; AB, alcian blue histochemistry for proteoglycans; DCN, immunohistochemistry for decorin; KER, immunohistochemistry for keratocan; LUM, immunohistochemistry for lumican. Top images correspond to histological microphotographs, whereas the signal quantification is represented, for those markers showing positive signal, in the lower panel. Scale bars: 50 μm