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. 2024 Oct 1;165(12):2794–2803. doi: 10.1097/j.pain.0000000000003421

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Copyright © 2024 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain.

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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Figure 1. — Visualization and analysis of activated neuronal populations in TG of Pirt-GCaMP3 Mice. (A) Representative image of whole TG captured using in vivo Pirt-GCaMP3 Ca²⁺ imaging of intact TG. The locations of neuronal cell bodies for V1 (ophthalmic), V2 (maxillary), and V3 (mandibular) are indicated. (B) Histogram depicting the cell number distribution according to cell diameter (μm) (n = 3 mice). (C) Graph showing the cell numbers categorized into 3 groups based on cell diameter: small (<20 μm), medium (20-25 μm), and large (>25 μm) (n = 3 mice). (D) Pie chart representing the percentage composition of each cell diameter size group (n = 3 mice). (E) Representative image of spontaneous activities captured through in vivo Pirt-GCaMP3 Ca ²⁺ imaging of intact TG. White arrowheads point to spontaneously activated neurons. (F) Representative heatmap displaying the spontaneously activated individual neurons in normal mice. (G) Bar graph showing the number of spontaneously activated neurons, categorized by cell diameter size group (n = 3 mice). (H) Pie chart showing the percentage composition of spontaneously activated neurons according to cell diameter size (n = 3 mice). S: small-diameter TG neurons (<20 μm); M: medium (20-25 μm); L: large (>25 μm). Error bars indicate SEM. TG, trigeminal ganglion.