Table 1.
Oligo Name | Sequence | Expected PCR Band Size |
---|---|---|
sg1 | ATGAAGGGGCAGAGACATCA | NA |
sg2 | CCTCCCTTCACAGCAAGGTA | NA |
CLN3 F1 | GGATGAATTAGATGGAGATTGAGG | Deleted: 600–800 bp |
CLN3 R1 | CTCATCCTACTTCTAATCACCTTG | Undeleted: 1529 bp |
CLN3 F2 | TCTGTCTCTACGGCTGCTGTGC | Deleted: 578 bp |
CLN3 R2 | GAACACCAGGTTGAGGCACTGC | Undeleted: 795 bp |