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. 2024 Nov 13;65(13):23. doi: 10.1167/iovs.65.13.23

Table 1.

List of CRISPR Guides and Primers Used to Confirm Deletion of Exons 7 and 8 in the CLN3 Gene

Oligo Name Sequence Expected PCR Band Size
sg1 ATGAAGGGGCAGAGACATCA NA
sg2 CCTCCCTTCACAGCAAGGTA NA
CLN3 F1 GGATGAATTAGATGGAGATTGAGG Deleted: 600–800 bp
CLN3 R1 CTCATCCTACTTCTAATCACCTTG Undeleted: 1529 bp
CLN3 F2 TCTGTCTCTACGGCTGCTGTGC Deleted: 578 bp
CLN3 R2 GAACACCAGGTTGAGGCACTGC Undeleted: 795 bp