| Strain, strain background (Mus musculus) |
Wildtype C57BL/6 mice (WT) |
Jackson Laboratory (West Grove, PA) |
Ref# 000664 |
|
| Strain, strain background (Mus musculus) |
Ccr2RFP (Ccr2–/–) |
Jackson Laboratory |
Ref# 017586 |
|
| Strain, strain background (Bacteria) |
Chromobacterium violaceum (C. violaceum) |
ATCC (Manassas, VA) |
Ref# 12472 |
|
| Antibody |
Rat anti-mouse Ly6G monoclonal (IA8) in BV421 |
BD Biosciences (Franklin Lakes, NJ) |
Ref# 562737 |
1:300 (FC) |
| Antibody |
Rat anti-mouse monoclonal (FA-11) CD68 in FITC |
BioLegend (San Diego, CA) |
Ref# 137005 |
1:300 (FC) |
| Antibody |
Rabbit anti-C. violaceum polyclonal |
Cocalico Biologicals (Denver, PA) |
Custom polyclonal antibody |
1:2000 (IHC, IF) |
| Antibody |
Rat anti-mouse Ly6G monoclonal (IA8) |
BioLegend |
Ref# 127601 |
1:300 (IHC) |
| Antibody |
Rabbit anti-mouse CD68 polyclonal |
Abcam (Waltham, MA) |
Ref# ab125212 |
1:200 (IHC) |
| Antibody |
Rat anti-mouse CD68 monoclonal (FA-11) in Alexa Fluor 488 |
Abcam |
Ref# ab201844 |
1:100 (IF) |
| Antibody |
Rat anti-mouse Ly6G monoclonal (IA8) in Alexa Fluor 647 |
BioLegend |
Ref# 127610 |
1:100 (IF) |
| Antibody |
Rabbit anti-mouse MCP1 (CCL2) polyclonal |
Abcam |
Ref# ab315478 |
1:100 (IF) |
| Antibody |
Goat anti-rabbit secondary polyclonal in Alexa Fluor 594 |
Invitrogen (Waltham, MA) |
Ref# A32740 |
1:1000 (IF) |
| Commercial assay or kit |
Avidin/Biotin Blocking Kit |
Vector Laboratories (Newark, CA) |
Ref# SP-2001 |
|
| Commercial assay or kit |
SignalStain Boost IHC Detection Reagent (HRP, Anti-Rabbit) |
Cell Signaling (Danvers, MA) |
Ref# 8114 |
|
| Commercial assay or kit |
ImmPRESS HRP Goat Anti-Rat Detection Kit |
Vector Laboratories |
Ref# MP-7404 |
|
| Commercial assay or kit |
DAB Substrate Kit, HRP |
Vector Laboratories |
Ref# SK-4100 |
|
| Commercial assay or kit |
H&E Stain Kit (Modified Mayer’s Hematoxylin and Bluing Reagent) |
Abcam |
Ref# ab245880 |
|
| Commercial assay or kit |
MCP-1/CCL2 Mouse Uncoated ELISA Kit |
Thermo Scientific (Waltham, MA) |
Ref# 88-7391-22 |
|
| Chemical compound, drug |
Reparixin |
MedChemExpress (Monmouth Junction, NJ) |
Ref# HY-15251 |
|
| Software, algorithm |
RStudio |
Posit PBC (Boston, MA) |
|
|
| Software, algorithm |
FlowJo |
BD Biosciences |
|
|
| Software, algorithm |
Prism 9 |
GraphPad (Boston, MA) |
|
|
| Software, algorithm |
Fiji |
ImageJ (Burleson, TX) |
|
|
| Other |
Collagenase Type IV |
Gibco |
Ref# 17104019 |
Tissue dissociation media |
| Other |
1× DMEM, +4.5 g/l D-Glucose, +L-Glutamine, +110 mg/l Sodium Pyruvate |
Gibco |
Ref# 11995-065 |
Cell culture media |
| Other |
1× RPMI Medium 1640, +L-Glutamine |
Gibco |
Ref# 11875-093 |
Cell culture media |
| Other |
PenStrep +10,000 units/ml Penicillin, +10,000 µg/ml Streptomycin |
Gibco |
Ref# 15140-122 |
Antibiotics |
| Other |
HyClone Characterized Fetal Bovine Serum |
Cytiva (Marlborough, MA) |
Ref# SH30396.03 |
Cell culture media |
| Other |
1× DPBS, -Calcium Chloride, -Magnesium Chloride |
Gibco |
Ref# 14190-144 |
Cell culture media |
| Other |
70 µm Cell Strainers |
Genesee Scientific (El Cajon, CA) |
Ref# 25-376 |
Tissue dissociation reagent |
| Other |
40 µm Cell Strainers |
Genesee Scientific |
Ref# 25-375 |
Tissue dissociation reagent |
| Other |
Percoll |
GE Healthcare (Chicago, IL) |
Ref# 17-0891-01 |
Tissue dissociation reagent |
| Other |
1× RBC Lysis Buffer |
eBioscience |
Ref# 00-4333-57 |
Flow cytometry reagent |
| Other |
Falcon Round-Bottom Polystyrene Test Tubes |
Thermo Scientific |
Ref# 14-959-1A |
Flow cytometry tubes |
| Other |
Mouse BD Fc Block |
BD Biosciences |
Ref# 553142 |
Blocking reagent; used at 1 µg (FC), 2% (IF) |
| Other |
Intracellular Fixation & Permeabilization Buffer |
eBioscience |
Ref# 88-8824-00 |
Flow cytometry reagent |
| Other |
10% Neutral Buffered Formalin |
VWR (Radnor, PA) |
Ref# 16004–128 |
Histology reagent |
| Other |
16% Paraformaldehyde |
VWR |
Ref# 15710S |
Immunofluorescence reagent |
| Other |
Sucrose |
Sigma-Aldrich |
Ref# S1888 |
Immunofluorescence reagent |
| Other |
Tissue-Tek O.C.T. Compound |
Sakura (Torrance, CA) |
Ref# 4583 |
Immunofluorescence reagent |
| Other |
Epredia Xylene |
Fisher Chemical |
Ref# 99-905-01 |
Immunohistochemistry reagent |
| Other |
ImmEdge Pen |
Vector Laboratories |
Ref# H-4000 |
Immunohistochemistry reagent |
| Other |
Normal Goat Serum Blocking Solution, 2.5% |
Vector Laboratories |
Ref# S-1012 |
Immunohistochemistry reagent |
| Other |
SignalStain Antibody Diluent |
Cell Signaling |
Ref# 8112 |
Immunohistochemistry reagent |
| Other |
Permount |
Fisher Chemical |
Ref# SP15-100 |
Immunohistochemistry reagent |
| Other |
T-PER Tissue Protein Extraction Reagent |
Thermo Scientific |
Ref# 78510 |
Tissue dissociation reagent |
| Other |
Sulfuric Acid |
Ricca Chemical (Arlington, TX) |
Ref# 8310-32 |
ELISA Stop Buffer |
| Other |
Fluoroshield with DAPI |
Sigma-Aldrich |
Ref# F6057 |
Immunofluorescence reagent |
