Fig. 5. Single and multiplex PCR techniques targeting the amplification of virulence genes. (a): Lanes 1, 2, 3, 5, and 8 positive to Lt gene (450 bp), lanes 1 to 9 positive to st gene (190 bp), lanes 3, 7, and 8 positive to f5 gene (314 bp). (b): lanes 1, 2, 4, 5, 6, 7, and 8 positive to Lt gene (450 bp), lanes 1 to 9 minus 8 positive to st gene (190 bp), lanes 1 to 7 positive to f5 gene (314 bp). (c): lanes 1, 3, 4, 5, 7, and 8 positive to Lt gene (450 bp), lanes 1 to 9 minus 8 positive to st gene (190 bp), lanes 1 to 7 positive to f5 gene (314 bp); lane 6 positive for Lt gene (450 bp), the rest negative, lanes 1 to 8 positive for st gene (190 bp), lanes 1, 3, 4, 5, 7, and 8 positive for f 5 gene (314 bp) (d): lanes 1, 2, 8, and 9 positive to the Lt gene (450 bp), lanes 1 to 9 minus 7 positive to the st gene (190 bp), lanes 1 to 9 minus 1 and 7 positive to the f5 gene (314 bp). (e): lane 9 positive to Lt gene (450 bp), lanes 1 to 9 positive to st gene (190 bp), lanes 1 to 9 minus 6 positive to f 5 gene (314 bp). (f): lanes 1 to 9 positive to st gene (190 bp), lanes 1 to 5 positive to Lt gene (450 bp), lane 1 positive to f 5 (314 bp). Prevalence of amplified virulence genes of E. coli strains according to sex.