. 2024 Sep 30;14(9):2170–2180. doi: 10.5455/OVJ.2024.v14.i9.6

Table 1. Primer sequence for the identification of the virulence genetic profile of E. coli (Awad et al., 2020).

Pathotype	Generation	Primer sequence	PCR amplification (bp)
EPEC	MAR (eae)	F: TCAATGCAGTTCCGTTATCAGTT	482 bp
EPEC	MAR (eae)	R: GTAAAGTCCGTTACCCCCCCCCCCCCCCCAACCTG	482 bp
STEC	Stx1 (stx1),	F: CGATGTTACGGTTTGTGTGTGTTACTGTGTGTGACAGC	244 bp
	Stx1 (stx1),	R: AATGCCACGCTTCCCAGAATTG	244 bp
	Stx2 (stx2)	F: CCATGACAACGGACGGACAGCAGTT	779 bp
	Stx2 (stx2)	R: CCTGTCAACTGAGCAGCAGCAGCAGCACTTTTTG	779 bp
	HlyA (hlyA)	F: AGCTGCAAGTGCGGGGGGTCTG	569 bp
	HlyA (hlyA)	R: TACGGTTATGCCTGCAAGTTCAC	569 bp
ETEC	LT (lt)	F: GGGCACACACACAGATTATATATATACCGTGC	450 bp
	LT (lt)	R: CGGTTCTCTCTATATTATTATTATTCCCTGTT	450 bp
	STREET	F: ATTTTTTTTMTTTCTTGTATTRTCTT	190 bp
	STREET	R: CACCCCGGTACARGCAGGATT	190 bp
	Fimbria f5 (k99)	F: TATTATATATCTTAGGTGGTGGTGGTGGTATGG	314 bp
	Fimbria f5 (k99)	R: GGTATATCCTTTAGCAGCAGCAGCAGTATTTATTC	314 bp
EAEC	ESTE1 (astA)	F: TGCCATCATCATCAACACACACACAGTATATATATCCG	102 bp
EAEC	ESTE1 (astA)	R: ACGGCTTTTGTAGTCCTTCCTTCCAT	102 bp