Table 2. Primer sequence of rat IL-1β, IL-6, TNF-α, p38 and GADPH.
| Gene | Sequence (5′–3′) | Product size (bp) |
Annealing (°C) |
References |
|---|---|---|---|---|
| IL-1β | 5′- AGAATACCACTTGTTGGCT-3′ 5′- GTGTGATGTTCCCATTAGAC-3′ |
134 | 55 | NM_031512.2 |
| IL-6 | 5′- GAGCATTGGAAGTTGGGGTA-3′ 5′- TGATGGATGCTTCCAAACTG-3′ |
230 | 53 | NM_012589.2 |
| TNF-α | 5′- GAAGACAATAACTGCACCCA-3′ 5′- AACCCAAGTAACCCTTAAAGTC-3′ |
138 | 54 | NM_012675.3 |
| P38 | 5′-AGATAATGCGTCTGACGGG-3′ 5′-AGGGGATTGGCACCAATAAA-3′ |
139 | 58 | NM_031020.3 |
| GADPH | 5′- GAAGGTGAAGGTCGGAGT-3′ 5′- GAAGATGGTGATGGGATTTC–3′ |
172 | 55 | NM_001357943.2 |