Figure 7. In vitro meiotic progression of oocytes from Hfm1^+/+ and Hfm1^–/– females.

(A) Confocal microscopy images after culture for 19–23 hours. DAPI staining alone is shown beneath each merged image. Hfm1^+/+, representative images of the majority of oocytes; Hfm1^–/– top, images observed that are closest to those from Hfm1^+/+ females; Hfm1^–/– bottom, examples of anomalies that were rarely seen in the oocytes from Hfm1^+/+ females. Scale bar: 100 μm. (B) Percentage of oocytes in each meiotic stage. DO, spontaneously denuded oocytes before culture. MII* indicates that the spindle resembles MII but no polar body or chromosomes are seen outside the oocyte.