FIGURE 5.

Flubendazole inhibited GSC proliferation and induced its apoptosis. (A) The gradient administration showed decreased GSC proliferation, decreased the number and size of neurospheres, and increased depolymerization of neurospheres and increased cell death (200X, 1:100 μm); (B) Apoptosis by flow cytometry revealed significant apoptosis in both GSCs after administration (Flubendazole, 24 h); (C) After treatment with Flubendazole, a decrease in CD133 and SOX2 was observed; (D) After Flubendazole treatment, there was a significant increase in MDA levels in the cells; (E) Following treatment with Flubendazole, TFRC showed a significant increase, while xCT and GPX4 decreased.(Student's t‐test was used. *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001).