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. 2024 Oct 28;18:200293. doi: 10.1016/j.tvr.2024.200293

Fig. 1.

Fig. 1

Diagram of SV40 genome, SV40 enhancer and Ig enhancers used in this study.

A. Genome organization of SV40. SV40 LT transcription unit integrated to Ramos, UO-31 and DT40 genome is indicated with rectangle with dashed lines. NCRR: non-coding regulatory region, LT: large tumor antigen, sT: small tumor antigen, 17 kT: 17 kDa protein, polyA: SV40 polyA signal, agno: agno protein: VP: viral structural proteins. Dashed lines to panel B indicate the SV40 enhancer region measured with GFP loss assay.

B. SV40 enhancer region and its modifications measured in GFP loss assays. Transcription factor binding sites which are important for SHM targeting in Ig enhancers are indicated in different colors.72bp: 72 base pair repeat sequence.

C. TFBS composition of chicken and human Igλ enhancers.

D. Sequence of SV40 enhancer. Locations of TFBSs are indicated in different colors (see panel B for coloring). 72bp repeats are highlighted with boxes. Changed nucleotides in mutated TFBSs are shown under the original sequence. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)