Extended Data Fig. 9. S6K1 rescues Il1a expression in double knockout MEFs.
a-b. Immunohistochemistry staining for RELA (a) and the corresponding quantification (b) of livers from Day 4 S6K1 WT (n = 5), KO (n = 8) mice and in Day 7 S6K1 WT (n = 7), KO (n = 6) mice. Scale bar, 100 μm. Data are expressed as mean ± SEM. Statistical significance was calculated using two-way analysis of variance with Tukey’s multiple comparison test. n denotes individual mice. c. Schematic of the rescue experiment performed in S6K1/2 WT or DKO MEFs transduced with the indicated vectors and undergoing replicative senescence. d-e. Representative immunofluorescence (IF) images (left) and quantification (right) of the percentage of cells positive for HA (d) or pS6S240/S244 (e) in S6K1/2 WT or DKO MEFs infected with the indicated vectors. Scale bar: 100 µm. Data represents mean ± SEM (n = 3 for all groups, except for KR n = 6). V, vector; wt, HA-S6K1 WT; KR, HA-S6K1 K100R. Ordinary one-way ANOVA. (Sidak’s multiple comparisons test). f. mRNA expression levels of Il1a in S6K1/2 WT or DKO MEFs with the indicated vectors undergoing replicative senescence measured by qRT-PCR. Data represents mean ± SEM (n = 4 for all groups, except for KR n = 9). Statistical significance was calculated using one-way ANOVA (Sidak’s multiple comparisons test). n represents biological replicates.