Extended Data Fig. 10. Confirmation of deletion in hepatocyte-specific or myeloid-specific S6K1/S6K2 knockout mice.

Hydrodynamic tail vein injection (HDTVi)-based co-delivery of an Nras^G12V transposon construct and a transposase expressing vector into mouse livers (day 0). Mice were sacrificed 4 or 7 days following HDTVi to assess senescence surveillance. Hepatocyte-specific S6K1/S6K2 (a) or myeloid-specific S6K1/S6K2 (b) KO mice or the floxed controls were used. a. Immunohistochemistry images for pS6^S240/S244 staining in hepatocyte-specific S6K1/S6K2 WT or KO mice. Scale bar: 100 µm. b. Immunofluorescence staining for F4/80 or pS6^S240/S244 staining in myeloid-specific S6K1/S6K2 WT or KO mice. Scale bar: 100 µm. Both (a) and (b) were single experiments with the n numbers indicated in Fig. 8.