Fig. 7. S6K1 regulates senescence surveillance.
a, Experimental scheme. HDTVi-based co-delivery of an NrasG12V transposon construct and a transposase expression vector into mouse livers (day 0). Mice were euthanized 4 d or 7 d after HDTVi to assess senescence surveillance. b–i, Immunohistochemistry staining for NRAS (b), MHC-II (d), CD68 (f) and CD3 (h) and the corresponding quantification (c,e,g,i) of livers from day 4 S6K1 WT (n = 7) and KO (n = 8) mice and in day 7 S6K1 WT (n = 7) and KO (n = 6) mice. Scale bar, 100 μm. j,k, In situ hybridization for Il1b mRNA (j) and quantification (k) of livers from day 7 S6K1 WT (n = 5) and KO (n = 5) mice. Scale bar, 50 μm. l,m, Immunohistochemistry staining for pIRF3S396 (l) and quantification (m) of livers in day 4 S6K1 WT (n = 6) and KO (n = 8) mice. Scale bar, 50 μm. Data are expressed as mean ± s.e.m. Statistical significance was calculated using two-way ANOVA with Tukey’s multiple comparison test (c,e,g,i) or by two-tailed Student’s t-test (k,m). n denotes individual mice.