Extended Data Fig. 1. S6K1 deletion attenuated age-induced obesity and ‘inflammaging’ in the liver.
S6K1 wild-type (WT) and knockout (KO) mice were aged for 600 days. a. Representative photograph of 600-day-old S6K1 WT (left) and KO (right) mice. b. Body weight (grams) at 600 days of S6K1 WT (left; n = 20) and KO (right; n = 13) mice. c. Epididymal white adipose tissue (eWAT) weight (grams) at 600 days of S6K1 WT (left; n = 8) and KO (right; n = 8) mice. d-e. F4/80 staining for resident Kupffer cells (d) and quantification (e) of livers from young S6K1 WT (90 days; n = 6), old S6K1 WT (600 days; n = 7) and old S6K1 KO (600 days; n = 8) mice. f-g. CD4 staining for T-helper cells (f) and quantification (g) of livers in young S6K1 WT (90 days; n = 5), old S6K1 WT (600 days; n = 8) and old S6K1 KO (600 days; n = 8) mice. h-i CD42b staining for platelets (h) and quantification (i) of livers in young S6K1 WT (90 days; n = 5), old S6K1 WT (600 days; n = 7) and old S6K1 KO (600 days; n = 8) mice. Data are expressed as mean ± SEM. Statistical significance was calculated using either a two-tailed Student’s t-test (b-c) or a one-way analysis of variance with Tukey’s multiple comparison test (e, g, i). n denotes individual mice. Scale bar, 100 μm. Data are expressed as mean ± SEM. Statistical significance was calculated using. n denotes individual mice.