Extended Data Fig. 2. Senolytics blunt the ability of aged bone marrow to induce senescence propagation to young recipients.
a, Outline of the studies (male mice). Old mice (16 m, male) were treated with Vehicle or D + Q for 5months. After 5 months, bone marrow was isolated from mice and then transplanted into young mice (3 m, male) (ABMT, DQ-ABMT). b, Immunofluorescence staining of γH2A.X in femoral bone sections (scale bar, 100μm; n = 3 mice; 5 ~ 6 images per mouse). c, Representative SA-β-gal staining of bone marrow in aged mice after DQ administration (scale bar, 250μm; n = 3 mice; 5 ~ 6 images per mouse). d-e, The protein levels of senescence-associated markers in muscle and quantitative analysis (n = 3 mice). f-g, Gene expression of senescence-associated markers in liver, muscle and adipose tissue of young mice receiving bone marrow from DQ-treated aged mice (n = 4 mice). h, Representative images of SA-β-gal staining in the liver (scale bar, 50μm; n = 4 mice; 5 ~ 6 images per mouse) and brain (scale bar, 50μm; n = 5 mice; 5 ~ 6 images per mouse). i-j, The AUC data for GTT and ITT were calculated, respectively (n = 6 mice). k, The mRNA levels of G6Pase, PEPCK, and PGC-1a in the liver (n = 4 mice). l, Gene expression of FASN and ACC1 in liver (n = 4 mice), and the LW/BW of young recipients (n = 6 mice). m-n, Western blot analysis of phosphorylated key molecules of insulin pathway in the liver and muscle, and their quantitative data (n = 3 mice). o, The mRNA levels of C-fos, Psd95, Foxo6 and Gfap in the brain (n = 3 mice). p-q, Immunofluorescence detection of GluR-1 in the brain and quantitative analysis (scale bar, 50μm; n = 5 mice; 5 ~ 6 images per mouse). Data are presented as mean ± SEM. *P < 0.05; **P < 0.01, ***P < 0.001; #P < 0.0001 were determined using two-tailed t-test in b-c and one-way ANOVA followed by Tukey’s multiple comparison test in d-q.