Extended Data Fig. 6. Mt-mKeima mitophagy flux assay in pentaKO cells rescued with OPTN and in presence or absence of NAP1/SINTBAD.
(a-b) pentaKO (5KO) and NAP1/SINTBAD DKO/5KO (clones #20 and #26)) expressing BFP-Parkin, GFP-OPTN and mt-mKeima were left untreated and treated with O/A for indicated time points and mitophagy flux was measured by flow cytometry (A) and quantified (B) (mean ± s.d) (n = 3 biologically independent experiments). Two-way ANOVA with Tukey’s multiple comparisons test was performed. *P < 0.05. ns, not significant. Source numerical data, including exact P values, are available in source data.