Extended Data Fig. 7. Mitophagy flux assay for wild-type and TBK1-binding deficient NAP1.
(a-b) Wild-type HeLa cells expressing BFP-Parkin and mt-mKeima with and without overexpression of NAP1 wild-type (WT) or TBK1-binding deficient mutant (L226Q/L233Q) (ΔTBK1), were left untreated or treated with O/A for indicated time points and mitophagy flux was measured by flow cytometry (A) and quantified (B) (mean ± s.d) (n = 3 biologically independent experiments). Two-way ANOVA with Tukey’s multiple comparisons test was performed. *P < 0.05, **P < 0.005, ***P < 0.001, ****P < 0.0001. ns, not significant. Source numerical data, including exact P values, are available in source data.